Biotechnology Journal Volume 5 Issue 1, Pages 17 - 23
Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice
Florian Krammer 1, Sabine Nakowitsch 2, Paul Messner 3, Dieter Palmberger 1, Boris Ferko 2, Reingard Grabherr, Dr. 1 *
1Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
2Avir Green Hills Biotechnology AG, Vienna, Austria
3Department of Nanobiotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture-based production systems and virus-like particle (VLP)-based technologies have been established. We developed swine-origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co-expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI-TN5B1-4 (High FiveTM). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses.